Amplifying Large AT rich amplicons with Pfu type polymerases (Allan)

This is a protocol for amplifying very large amplicons that are high in AT.  I developed in order to amplify the 8.2 Kb region containing the promoter of Arabidopsis FT which is approximately 70% AT.

In my hands it worked reliably with an 8 Kb amplicon (DeBono notebook 1, July 2010) but can be easily modified for longer products using more dNTP and optimized template concentration.

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