Cutting a slice from an agarose gel in order to size select DNA or to isolate a PCR product etc. is standard practice in lab genetics. This post is about how I do it in our lab, (as of Feb 2012).
There are three features of my current method that may be unfamiliar to you even if you’ve done this sort of thing before:
- The Dark Reader transilluminator.
- SYBR-Gold.
- Sigma X-Tracta gel punches.