NAPS, the UBC service we use for Sanger sequencing, charges 2 CAD for purifying a sequencing reaction; especially if submitting several reactions, there is therefore a reasonably big incentive in doing the purification ourselves. Continue reading
SPRI beads cleaning is one of the most repetitively used step during library preparations and probably the step where most of us lose a lot of precious DNAs.
Losing DNA scared me so much (because it can be observable) that I hesitated a lot before trying to use beads to concentrate genomic DNA, because usual rate of recovery are ~50%.
Hopefully with some practice and a lot of patience, it is possible to reach 90% recovery. How to lose as little DNA as possible? Here are some guidelines: Continue reading