Here is a seed sterilization protocol that surprised me – No fungus visible after 4 -5 days of growth on nutritious media.
In 100 ml distilled water mix the following
1 g sparkleen soap powder (dish washing stuff) –> This is the top end for sparkleen. You may need to use less.
2 mL bleach (final concentration = 2%)
2 ml PPM. (final concentration = 2%)
Sterilize in 15 ml tubes.
Rinse seeds with autoclaved water 3x.
Results: Normally the first image would contain many fungal blooms. Not so in the images below.
Friday November 21st