DSN depletion for GBS libraries

This step is mentioned in our current GBS protocol, but I forgot to upload it until now. It’s basically the same as the WGS one, with minor changes. I am attaching a couple of bioanalyzer plots of the same library before and after DSN treatment. The sharp peaks/thick bands disappearing after the DSN treatment are likely chloroplast fragments.

Size-selected GBS, before depletion

A size-selected GBS library before…

Size-selected GBS, after depletion

…and after depletion

Depletion of repetitive sequences – GBS libraries