We have had some luck with paralyzing worms with a BDM (2,3-butanedione monoxime) levamisole mix. I still have a lot of movement with just 10mM levamisole, but this mix is great.
We make 0.3M BDM (0.3M BDM, 10mM NaHEPES, pH 7.2). Then we mix 3 parts 0.3M BDM with 1 part 10mM levamisole.
The abstract for using BDM was published by Miriam Goodman and Marty Chalfie in Worm Breeder’s Gazette: 15(3): 9 (June 1, 1998)
http://elegans.swmed.edu/wli/%5Bwbg15.3p9%5D/
Notes to the ShenLab: Both BDM and Lvm stocks are found on the top shelf in the -20ºC in the computer room.