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Strain Integration

I. Prep work(3 days before starting the protocol, ie “day -3”)

  1. Place request for plates
    1. Request “big” agarose plates for mutagenesis (no bacteria on plates, request them for day -1 so that you can use them on day 0)
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Lab Protocols

Injection

A couple good pictures and a movie on injecting. http://130.15.90.245/microinjection.htm

1. Pull needles using program #88

2. Spin 10ul of DNA at max for 20′ (less will probably work)