I. Prep work(3 days before starting the protocol, ie “day -3”)
- Place request for plates
- Request “big” agarose plates for mutagenesis (no bacteria on plates, request them for day -1 so that you can use them on day 0)
I. Prep work(3 days before starting the protocol, ie “day -3”)
A couple good pictures and a movie on injecting. http://130.15.90.245/microinjection.htm
1. Pull needles using program #88
2. Spin 10ul of DNA at max for 20′ (less will probably work)