What We've Done

Natural Competence
Published Research
Reviews and Book Chapters
Methods
Research in Other Areas
Arsenic
The Hotspot Paradox
Protist Evolution
Quorum Sensing
Evolution of Sex
Pedagogy

Ongoing research on natural competence:

In press:

Sinha S, Mell JC and Redfield RJ. 2013. The availability of purine nucleotides regulates natural competence by controlling translation of the competence activator Sxy. Molecular Microbiology. doi:10.1111/mmi.12245. PDF

Published research:

Sinha S, Mell JC and Redfield RJ. 2012. 17 CRP-S-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology. 194(19): 5245-54. PDF
 
 
Mell JC, Hall IM and Redfield RJ. 2012. Defining the DNA Uptake Specificity of Naturally Competent Haemophilus influenzae cells. Nucleic Acid Research. 40(17):8536-49. PDF

Kristensen BM,
Sinha S, Boyce JD, Bojesen AM, Mell JC and Redfield RJ. 2012. Natural Transformation by Gallibacterium anatis. Applied and Environmental Microbiology. 78(14): 4914-22. PDF

Sinha S and Redfield RJ. 2012. Natural DNA Uptake by Escherichia coli. PLoS One. 7(4): e35620. PDF
 
Mell JC, Shumilina S, Hall IM and Redfield RJ. 2011. Transformation of Natural Genetic Variation into Haemophilus influenzae Genomes. PLoS Pathogens. 7: e1002151. PDF
 

Maughan H, Wilson LA and Redfield RJ. 2010. Bacterial DNA Uptake Sequences Can Accumulate by Molecular Drive Alone. Genetics. 186: 613-627 PDF

Redfield RJ. 2009. Looking to bacteria for clues. Science. 325(5943): 946
PDF

Sinha S,
Bossé J, Schippers T, Kroll JS, Redfield RJ and Langford P. 2009. Natural competence in strains of Actinobacillus pleuropneumoniae. FEMS Microbiology Letters. 298(7):124-30 PDF

Sinha S, Cameron ADS and Redfield RJ. 2009. Sxy induces a CRP-S regulon in Escherichia coli. Journal of Bacteriology.
191(6):5180-95 PDF  Supplementary material

Maughan H and Redfield RJ. 2009. Extensive variation in natural competence in Haemophilus influenzae. Evolution.
63(7):1852-66 PDF

Findlay WA and Redfield RJ. 2009. Coevolution of DNA Uptake Sequences and Bacterial Proteomes. Genome. Biol Evol. 5(1):45-55. PDF

Maughan H and Redfield RJ. 2009. Tracing the evolution of competence in Haemophilus influenzae. PLoS One 4(6):e5854. PDF

Cameron ADS and Redfield RJ. 2008. CRP binding and transcription activation at CRP-S sites. Journal of Molecular Biology 383(2):313-23. PDF


Cameron ADS, Volar M, Bannister LA and Redfield RJ. 2008. RNA secondary structure regulates the translation of sxy and competence development in
Haemophilus influenzae
. Nucleic Acids Research 36(1):10-20.  PDF


Cameron ADS and Redfield RJ. 2006. Non-canonical CRP sites control competence regulons in Escherichia coli and many other gamma-proteobacteria. Nucleic Acids Research 36(1) : 10-20. PDF Supplementary tables

Redfield RJ, Findlay WA, Bossé J, Kroll JS, Cameron ADS and Nash JHE. 2006. Evolution of competence and DNA uptake specificity in the Pasteurellaceae. BMC Evolutionary Biology 6:82. PDF

Redfield RJ, Cameron ADS, Qian Q, Hinds J, Ali TR, Kroll JS and Langford P. 2004. A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae. 2005. Journal of Molecular Biology 347: 735-747. PDF

Bakkali M, Chen T-Y, Lee H-C and
Redfield RJ. 2004. Evolutionary stability of DNA uptake sequences in the Pasteurellaceae. Proceedings of the National Academy of Sciences, USA 101: 4513-4518. PDF

Wang Y, Goodman S,
Redfield RJ and Chen C. 2002. Natural transformation and DNA uptake signal sequences in Actinobacillus actinomycetemcomitans. Journal of Bacteriology 184: 3442-3449. PDF

Macfadyen LP, Chen D, Vo HC, Liao D, Sinotte R and Redfield RJ. 2001. Competence development by Haemophilus influenzae is regulated by the availability of nucleic acid precursors. Molecular Microbiology 40: 700-7. PDF

Ma C and
Redfield RJ. 2000. Point mutations in a peptidoglycan biosynthesis gene cause competence induction in Haemophilus influenzae. Journal of Bacteriology 182:3323-3330. PDF

Macfadyen LP, Ma C and Redfield RJ. 1998. A 3':5'-cAMP phosphodiesterase modulates cAMP levels and optimizes competence in H. influenzae Rd. Journal of Bacteriology 180: 44-1-4405. PDF

Macfadyen LP. 2000. Regulation of competence development in Haemophilus influenzae. Journal of Theoretical Biology 207: 349-59. PDF

Redfield RJ, Schrag MR and Dean AM. 1997. The evolution of bacterial transformation: sex with poor relations. Genetics 146:27-38. PDF

Macfadyen LP, Dorocicz I, Reizer J, Saier Jr. MH and Redfield RJ. 1996. Regulation of competence development and sugar utilization in
Haemophilus influenzae
Rd by a phosphoenolpyruvate:fructose phosphotransferase system. Molecular Microbiology 21 (5):941-952. PDF

Macfadyen LP and
Redfield RJ. 1996. Life in Mucus: Sugar metabolism in Haemophilus influenzae. Research in Microbiology 147: 541-551 (this paper is an invited contribution to a single-topic issue). PDF

Redfield RJ. 1993. Evolution of natural transformation: Testing the DNA repair hypothesis in Haemophilus influenzae and Bacillus subtilis. Genetics 133:755-761. PDF

Williams P, Bannister L and Redfield RJ. 1994 The Haemophilus influenzae sxy-1 mutation is in a newly identified gene essential for competence. Journal of Bacteriology 176 (22) 6789-6794. PDF

Dorocicz I, Williams P, and
Redfield RJ. 1993. The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence. Journal of Bacteriology 175:7142-49. PDF

Redfield RJ. 1993. Genes for breakfast: the have-your-cake-and-eat-it-too of natural transformation. J. Hered. 84: 400-404 (Proceedings of AGA Symposium, 1992).

Redfield RJ. 1991. sxy-1, a Haemophilus influenzae mutation causing greatly enhanced spontaneous competence. Journal of Bacteriology 173: 5612-5618. PDF

Redfield RJ. 1991. Bacterial mating preferences. Nature 352: 25-26.

Tomb J-F, Barcak G, Chandler M,
Redfield RJ and Smith HO. 1989. Transposon mutagenesis, characterization, and cloning of transformation genes of Haemophilus influenzae Rd. Journal of Bacteriology 171: 3796-3802. PDF

Lee JJ, Smith HO and
Redfield RJ. 1989. Organization of the Haemophilus influenzae Rd genome. Journal of Bacteriology 171: 3016-3024. PDF

Redfield RJ. 1988. Evolution of bacterial transformation: is sex with dead cells ever better than no sex at all? Genetics 119: 213-221. PDF

Reviews and Book Chapters:

Maughan H, Sinha S, Wilson L, Redfield R. 2008. Competence, DNA Uptake and Transformation in the Pasteurellaceae. Chapter in Pasteurellaceae: Biology, Genomics and Molecular Aspects, Caister Academic Press, ISBN: 978-1-904455-34-9. PDF

Redfield RJ. 2001. Do bacteria have sex? Nature Reviews Genetics 2:634-9. PDF

Redfield RJ. 2001 Transformation. Encyclopedia of Genetics, Academic Press.

Redfield RJ. 2001 Transformation and Transfection. Two entries in the Encyclopedia of Genetics, Academic Press. PDF

Redfield RJ. 2002 Haemophilus influenzae, Genetics. Entry in Encyclopedia of Microbiology, Academic Press.

Redfield RJ. 1997. The problem of the evolution of sex. Proceedings of a NASA Life Sciences Workshop on Evolution: a molecular perspective. Woods Hole, October 1997.

Redfield RJ. 1999. A truly pluralistic view of sex and recombination. Journal of Evolutionary Biology 12: 1043-1046 PDF

Redfield RJ. 1996. The Haemophilus influenzae genome: after sequencing, then what? MedScape/Women's Health , June 1996 (a peer-reviewed on-line journal, available at http://www.medscape.com). Mooers 1996.

Methods:

Poje G and
Redfield RJ. 2002. General Methods for culturing Haemophilus influenzae, and Transformation of Haemophilus influenzae. Two chapters in Molecular Methods for Haemophilus influenzae:, M. Herbert, Editor, Humana Press. Growth PDF, Transformation PDF

Williams P, Hung WL, and
Redfield RJ. 1996. Cell transfer by filtration: evaluation of protocols for transformational competence. FEMS (Federation of European Microbiology Societies) Microbiology Letters 137:183-187.


Research in other areas:

Arsenic:

Reaves ML, Sinha S, Rabinowitz JD, Kruglyak L and Redfield RJ. 2012. Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells. Science. 337(6093): 470-3. PDF

Redfield RJ. 2011. Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus". Science. 332. PDF

The Hotspot Paradox:

Pineda-Krch M and Redfield RJ. 2005. Persistence and loss of meiotic recombination hotspots. Genetics 169:2913-2928. PDF

Boulton A, Myers R and Redfield RJ. 1997. The hotspot conversion paradox and the evolution of meiotic recombination. Proceedings of the National Academy of Sciences, USA 94:8058-8063. PDF

Protist Evolution:

Dacks JB, Silberman JD, Simpson AG, Moriya S, Kudo T, Ohkuma M and Redfield RJ. 2001. Oxymonads are closely related to the excavate taxon Trimastix. Molecular Biology and Evolution 18:1034-44. PDF

Dacks JB and
Redfield RJ. Phylogenetic placement of Trichonympha. Journal of Eukaryotic Microbiology 45: 445-447.

Quorum Sensing:

Redfield RJ. 2002. Is quorum sensing a side effect of diffusion sensing? Trends in Microbiology 10:365-370. PDF

Evolution of Sex:

Redfield RJ. 1994. Male mutation rates and the cost of sex for females. Nature 369: 145-147. PDF


Pedagogy:

Redfield RJ. 2012. "Why do we have to learn this stuff?" - A New Genetics For 21st Century Students. PLoS Biology. 10(7): e1001356. PDF

Redfield RJ, Mayer-Smith JA and Griffiths A. 1997, Stuck on the Web. Genetics Society of Canada Bulletin. 28(1): 23.

Yazon J, Mayer-Smith JA and Redfield RJ. 2002. Does the medium change the message? The impact of a web-based genetics course on university students’ perspectives on learning and teaching. Computers and Education 38: 267-285.

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