Paramecium culture procedure:

The following instructions, provided by Joey Courchesne, are for 1L of paramecium culture

Materials needed: 2L flask, 2.5g cerophyl powder (wheat grass powder), 3/4 g of dibase sodium phosphate powder, klebsiella starter culture, large jar (<2L), and a hotplate.

1. Fill flask with 1L of distilled water
2. Measure out 2.5g of wheat grass powder and 3/4 of a gram of sodium phosphate and add to water
3. Bring mixture to a boil
4. Allow mixture to cool to room temperature
5. [optional step] Filter cooled mixture through cotton or nitex to filter out wheat grass particles
6. [optional step] Autoclave cooled and filtered mixture
7. Inoculate mixture with klebsiella medium (cut off a chunk of the bacteria rich agar and add straight to the mixture; the piece of agar should be about the size of your thumbnail)
8. Incubate inoculated mixture overnight (8-12 hours) @ 37 degrees. The high temperature is necessary for proper bacterial bloom
9. Pour mix into the large jar and add paramecium culture; or add mix directly to existing paramecium culture for continued rapid growth
10. Allow paramecium population to expand for 1-3 days
11. Ripe culture should have a paramecium population visible as a "cloud" in the jar. Sufficient population size can be confirmed with a dissection microscope where paramecium presence should be very strongly evident.
12. Replenish culture with new food (steps 1-8) every 1-4 weeks as needed