Normalize/quantify your WGS libraries

Regardless of what method you use to make your Illumina libraries, if you added barcodes or indices you will need to normalize them before pooling (or otherwise have probably very uneven coverage). Or, you might want to know the exact molarity of your library before sending it for sequencing (although the need for that in our case is debatable, see later). The most accurate way to do both is probably by qPCR. Continue reading