Here is the long awaited updated GBS protocol.
PROTOCOL ->>>> GBSv2.0
There are three main changes from the previous protocol.
-After digestion and ligation, all the product is kept so more attempts can be made at the PCR.
-The PCR uses Phusion Taq, has longer extension times and one additional cycle with more primers/Taq.
-Size selection is done using AMPure beads instead of gel extraction.