Electro-Competent EHA-105 Agrobacterium Cells for Transformation

The following protocol is for producing electro-competent cells of agro-bacterium.  Currently A. tumefaciens agropine-type strain EHA-105 (Hood et al.,1995) is used for transformation of sunflower and arabidopsis. Harvesting new competent cells takes 4 days to complete with the 4th day being the most labour intensive. It’s advised to begin early in the morning on the 4th day as the length of culture, purifying, and aliquoting can take several hours.

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