Illumina Sequencing Adapters (Dan E.)

I’ve been writing posts about the various steps involved in making WGS sequencing libraries for the Biodiversity Centre’s Illumina HiSeq machine and I was tempted to try to explain what the adapters are for. Then I had the bright idea of seeing if somebody else had already done it. Somebody has.

First, here is a nice simple animation describing how the Illumina sequencing systems works courtesy of the Wellcome Trust.

Here is the Illumina version of the same thing at Youtube, (the one above is better).

Somebody called James Schiemer at Tufts University has made this nice pdf explaining what the bits of the Illumina adapters are for.

Dan E.