| Who we are | What we do | Contact Us |
|---|---|---|
| We are the C. elegans Gene Knockout Lab at the University of British Columbia in Vancouver. We're a small group of dedicated, tireless genetic and molecular biological professionals working with the free-living nematode Caenorhabditis elegans. There are currently nine of us (although one is really a water strider guy):
Don Moerman, Director Mark Edgley, Lab Manager Locke Rowe, Visiting Scientist Vinci Au, Technician Claudia Doell, Technician Lisa Fernando, Technician Erica Li-Leger, Technician Kiana Martin, Technician Viktorija Jucuite, Research Assistant Stephane Flibotte has become Head of Bioinformatics at the UBC Life Sciences Institute. | We are engaged in a systematic effort to create knockouts for each of the approximately 21,000 genes in the C. elegans genome. Our current efforts are focused on the remaining 4,000 genes with no known nonsense or indel alleles. To accomplish this, we are using a CRISPR-Cas9 Homology Directed Repair strategy (G3 9(1): 135-144, 2019; Genetics 201 No. 2: 449-458, October 1, 2015) to obtain precise out-of-frame deletions in targeted genes. This strategy allows us to pick high value target genes and to complete large gene families, including transcription factors and kinases. All data from this project is submitted to WormBase, and all strains may be obtained through the Caenorhabditis Genetics Center. We are currently accepting requests for CRISPR knockouts, subject to restrictions (a small number of requests per lab, and only genes for which no knockout mutation exists). Please contact Don Moerman for details and advice. |
Life Sciences Centre, UBC 2350 Health Sciences Mall Vancouver, BC, Canada V6T 1Z3 Dr. Don Moerman, Director moerman@zoology.ubc.ca 604-822-3365 Mark Edgley, Lab Manager edgley@mail.ubc.ca 604-822-0221 |