Sunflower seed sterilization

Hi all,

Here is a seed sterilization protocol that surprised me – No fungus visible after 4 -5 days of growth on nutritious media.

In 100 ml distilled water mix the following

1 g sparkleen soap powder (dish washing stuff) –> This is the top end for sparkleen.  You may need to use less.
2 mL bleach (final concentration = 2%)
2 ml PPM. (final concentration = 2%)

Sterilize in 15 ml tubes.

Rinse seeds  with autoclaved water 3x.

Results: Normally the first image would contain many fungal blooms.  Not so in the images below.

Friday November 21st



Monday November 26thWP_20131125_001