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Myoblast Migration
Mutation Detection:
- Gene Knockouts
- 2000 Genomes
Muscle Research:
- Myofilament Research
- Myoblast Migration
In C. elegans myoblasts arise from several different founder cell lineages. These cells initially arrange themselves in two rows along the left and right lateral midlines and at ~290 min of development they migrate dorsally and ventrally to form the four muscle quadrants present upon hatching. As the myoblasts migrate they are still dividing, as are many other cells around them. This means the cell-cell contacts of cells during migration varies from animal to animal. This situation creates an environment where the extracellular matrix (ECM) and cell surface contacts are in constant flux, which begs the questions as to how these cells navigate unerringly to their final destination. A number of ECM and cell surface components are known to affect cell positioning, migration and attachment in other organisms. The C. elegans homologues of these proteins are involved in the same processes, but during late development, and do not appear to have a role during myoblast migration. In an effort to identify the proteins involved in early myoblast migrations we are currently testing genes predicted to be in, or interacting with the ECM using RNAi knockdowns. A two-tiered approach involving fluorescent and 4-D microscopy is being used to identify RNAi treated animals for potential muscle migration and positioning defects.
Inactive Projects
- Expression Project