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Current Research Projects
Mutation Detection:
- Gene Knockouts
- 2000 Genomes

Muscle Research:
- Myofilament Research
- Myoblast Migration
Inactive Projects
- Expression Project

 

 
2000 Genomes
This is a collaborative project between the Genome Sciences Department in Seattle (R.H. Waterston, L. Hillier and J. Shendure) and the Moerman Lab. Over the next 18 months, starting April 1, 2010, we will build a collection of 1,500 to 2,000 mutagenized lines using various mutagens, including EMS, ENU and UV/TMP. Each line will be initially selected as F1 progeny using nicotine to screen for heterozygous unc-22 mutations, to insure that the chosen animal has been adequately exposed to the mutagen. The F2 generation will be screened in nicotine to select a non-Unc-22 animal so that the final line will have no obvious phenotype. Each line will be propagated clonally through 10 generations to drive background mutations to homozygosity, and will then be frozen for permanent storage.

Genomic DNA from each line will be subjected to whole-genome sequencing using Next Generation Illumina technology. From recent work in our lab (Flibotte et al., Genetics, in press), we expect that 2,000 lines will produce a resource with about 1million useful mutations (about 10 non-synonymous changes per gene). The sequence data will be deposited at WormBase and the strains will be made available through the Caenorhabditis Genetics Center (individually, and hopefully as entire frozen sets arrayed in 96-well format). We have made a good start on the project, with nearly 800 strains generated and frozen, and approximately 250 of these are currently in the sequencing queue at Washington University.

We imagine that researchers will be able to screen the whole library for phenotypes of interest, multiple alleles of genes, or for gene interactions via RNAi, chemical or environmental screening.